Journal: Lasers in Surgery and Medicine

Article Title: Effects of Nonablative Er‐ YAG Laser on Human Endometrial Stromal Cells (hESCs): A Pilot Study

doi: 10.1002/lsm.70020

Figure Lengend Snippet: The boxplots of the IGFBP‐1 secretions in the cultured media of human endometrial stromal cells (hESCs). Confluent hESC cultures were treated with E 2 (10 −8 M) (group E 2 ), E 2 (10 −8 M) + ethanol (0.1%) (group E 2 + S), E 2 (10 −8 M) + P 4 (10 −7 M) (group E 2 + P 4 ), Er‐YAG laser+ E 2 (10 −8 M) (group E 2 + L), Er‐YAG laser + E 2 (10 −8 M) + P 4 (10 −7 M) (group E 2 + P 4 + L) for 12, 24, 48, and 72 h. Only hESCs (incubated in serum‐free DMEM/F12) and hESCs + Er‐YAG laser (hESCs + L) groups were incubated for 12 and 72 h. IGFBP‐1 levels were quantified by ELISA in culture media and normalized to total cell protein ( n = 3, median (Q1–Q3). DMEM, Dulbecco modified Eagle medium; E 2 , estradiol; ELISA, enzyme‐linked immunosorbent assay; Er‐YAG, nonablative Erbium YAG; ESC, endometrial stromal cell; IGFBP‐1, insulin‐like growth factor‐binding protein‐1; P 4 , progesterone.

Article Snippet: To investigate the effect of the Er‐YAG laser on endometrial decidualization, we measured insulin‐like growth factor‐binding protein‐1 (IGFBP‐1) levels in conditioned media using ELISA kits (Elabscience; E‐EL‐H0442, Houston, TX, USA) [ ] with a sensitivity of 0.10 ng/mL and no reported cross‐reactivity or interference.

Techniques: Cell Culture, Incubation, Enzyme-linked Immunosorbent Assay, Modification, Binding Assay

Journal: Molecular human reproduction

Article Title: Decreased expression of NR4A nuclear receptors in adenomyosis impairs endometrial decidualization.

doi: 10.1093/molehr/gaw042

Figure Lengend Snippet: Figure 2 NR4A regulation of human endometrial stromal cell (hESC) decidualization in vitro. hESCs were transduced with Ad-LacZ or Ad-NR4A at a multiplicity of infection (MOI) of 25 or were treated with 0.5 mM 8-Br-cAMP and 1 µM MPA for 48 h. PRL (A) and IGFBP1 (B) mRNA levels were measured by real-time PCR. The results are presented as the mean ± SEM; n = 3 samples. * P < 0.05, ** P < 0.01, and *** P < 0.001 compared with Ad-LacZ. hESCs were infected with the indicated adenoviruses at a MOI of 50, followed by treatment with or without 8-Br-cAMP and MPA for an add- itional 4 days. Prolactin (C) and IGFBP-1 (D) released into the medium was measured by Enzyme Linked Fluorescent Assay (ELFA) and ELISA. The results are presented as the mean ± SEM; n = 5 samples. * P < 0.05, ** P < 0.01, and *** P < 0.001 compared with Ad-LacZ. hESCs were transfected with 50 nM of the indicated specific NR4A siRNA for 72 h, followed by treatment with or without 8-Br-cAMP and MPA for an additional 4 days. Prolactin (E) and IGFBP-1 (F) released into the medium were measured by ELFA and ELISA. The results are presented as the mean ± SEM; n = 4 sam- ples. *** P < 0.001 compared with siCTL. # P < 0.05 and ## P < 0.01 compared with siCTL plus 8-Br-cAMP and MPA. (G and H) Fluorescein isothio- cyanate labeled phalloidin was used to label actin filaments, and immunofluorescence was used to analyze the morphological transformation of hESCs.

Article Snippet: Detection of prolactin and IGFBP-1 levels in cultured supernatants Prolactin and IGFBP-1 levels in supernatants were measured using a Vidas prolactin kit (bioMérieux) and a human IGFBP1 ELISA kit (BOSTER).

Techniques: In Vitro, Transduction, Infection, Real-time Polymerase Chain Reaction, Fluorescence, Enzyme-linked Immunosorbent Assay, Transfection, Labeling, Transformation Assay

Journal: EMBO Molecular Medicine

Article Title: FGF21 gene therapy as treatment for obesity and insulin resistance

doi: 10.15252/emmm.201708791

Figure Lengend Snippet: The long‐term effects of FGF21 gene transfer on bones were studied by comparison of HFD‐fed mice treated with the highest dose (5 × 10 10 vg/mouse) of FGF21 vectors as young adults or adults with null‐injected, chow or HFD‐fed animals. A Total naso‐anal length. B Tibial length. C–O Micro‐computed tomography (μCT) analysis of the epiphysis (C–J) and the diaphysis (K–O) of tibiae obtained at the time of sacrifice, that is, when animals were 18 months of age, from HFD‐fed mice administered with either null or FGF21‐encoding AAV vectors. P, Q Circulating IGFBP1 (P) and IGF1 (Q) levels measured by ELISA. Data information: All data represent the mean ± SEM. In (A, P, Q), Young adults: AAV8‐hAAT‐null chow ( n = 10 animals), AAV8‐hAAT‐null HFD ( n = 8), AAV8‐hAAT‐FGF21 HFD 1 × 10 10 vg ( n = 9), and 5 × 10 10 vg ( n = 8). Adults: AAV8‐hAAT‐null chow ( n = 7), AAV8‐hAAT‐null HFD ( n = 7), AAV8‐hAAT‐FGF21 HFD 1 × 10 10 vg ( n = 7), 2 × 10 10 vg ( n = 8), and 5 × 10 10 vg ( n = 7). In (B–O), n = 4 animals/group. In (A, B, P, Q), data were analyzed by one‐way ANOVA with Tukey's post hoc correction. In (C–O), data were analyzed by unpaired Student's t ‐test. ** P < 0.01 and *** P < 0.001 versus the chow‐fed null‐injected group. HFD, high‐fat diet; BMD, bone mineral density; BMC, bone mineral content; BV, bone volume; BV/TV, bone volume/tissue volume ratio; BS/BV, bone surface/bone volume ratio; Tb.N, trabecular number; Tb.Th, trabecular thickness; Tb.Sp, trabecular separation.

Article Snippet: Serum FGF21, adiponectin, leptin, IGFBP1, and IGF1 were determined using the Mouse/Rat FGF‐21 ELISA kit (MF2100, R&D Systems), the Mouse Adiponectin ELISA kit (80569, Crystal Chem), the Mouse Leptin ELISA kit (90030, Crystal Chem), the IGFBP1 (Mouse) ELISA kit (KA3054, Abnova), and the m/r IGF‐I‐ELISA kit (E25, Mediagnost), respectively.

Techniques: Comparison, Injection, Micro-CT, Enzyme-linked Immunosorbent Assay